5S58

Tubulin-Z2856434826-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	104.280, 157.510, 179.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.140 - 2.300
R-factor	0.2106
Rwork	0.209
R-free	0.24620
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	72.140	72.140	2.360
High resolution limit [Å]	2.300	10.290	2.300
Rmerge	0.106	0.023	2.495
Rmeas	0.115	0.025	2.692
Total number of observations	895400
Number of reflections	131637	1644	9651
<I/σ(I)>	11.35	60.38	0.74
Completeness [%]	99.9	99.4	99.8
Redundancy	6.802	6.325	7.064
CC(1/2)	0.999	0.999	0.345

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine