5S57

Tubulin-Z2856434883-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	104.680, 159.270, 180.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.380 - 2.450
R-factor	0.2088
Rwork	0.207
R-free	0.24530
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	63.380	63.380	2.510
High resolution limit [Å]	2.450	10.960	2.450
Rmerge	0.099	0.026	2.926
Rmeas	0.108	0.028	3.165
Total number of observations	743498
Number of reflections	111197	1385	8129
<I/σ(I)>	10.63	51.85	0.64
Completeness [%]	99.8	99.1	99.7
Redundancy	6.686	6.344	6.903
CC(1/2)	0.999	0.999	0.264

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine