5S56

Tubulin-Z2856434783-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	104.560, 158.300, 180.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.660 - 2.250
R-factor	0.2061
Rwork	0.204
R-free	0.23900
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	62.660	62.660	2.310
High resolution limit [Å]	2.250	10.060	2.250
Rmerge	0.080	0.027	2.410
Rmeas	0.087	0.029	2.602
Total number of observations	969932
Number of reflections	141883	1754	10428
<I/σ(I)>	12.24	53.15	0.77
Completeness [%]	99.9	99.3	99.8
Redundancy	6.836	6.253	7.032
CC(1/2)	0.999	0.999	0.334

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine