5S52

Tubulin-Z50145861-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	105.090, 161.570, 174.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	118.670 - 2.830
R-factor	0.2414
Rwork	0.238
R-free	0.29690
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	118.670	118.670	2.900
High resolution limit [Å]	2.830	12.660	2.830
Rmerge	0.148	0.022	2.223
Rmeas	0.161	0.024	2.419
Total number of observations	457325
Number of reflections	69748	904	5209
<I/σ(I)>	9.96	54.82	0.88
Completeness [%]	97.3	98.6	99.9
Redundancy	6.557	6.128	6.431
CC(1/2)	0.998	0.999	0.317

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine