5S4W

Tubulin-Z1416571195-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	105.130, 160.080, 178.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.660 - 2.800
R-factor	0.2235
Rwork	0.221
R-free	0.26820
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	62.660	62.660	2.870
High resolution limit [Å]	2.800	12.520	2.800
Rmerge	0.114	0.024	2.947
Rmeas	0.124	0.027	3.180
Total number of observations	501514
Number of reflections	73656	939	5415
<I/σ(I)>	11.67	62.23	0.67
Completeness [%]	98.3	98.3	98.9
Redundancy	6.809	6.373	7.103
CC(1/2)	0.999	0.999	0.358

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine