5S4V
Tubulin-Z57040482-complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-14 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.91587 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.420, 158.060, 178.360 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.020 - 2.300 |
R-factor | 0.2066 |
Rwork | 0.205 |
R-free | 0.24220 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5lxt |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX (1.18.2_3874) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 63.020 | 63.020 | 2.360 |
High resolution limit [Å] | 2.300 | 10.290 | 2.300 |
Rmerge | 0.100 | 0.028 | 2.671 |
Rmeas | 0.109 | 0.031 | 2.883 |
Total number of observations | 891857 | ||
Number of reflections | 131168 | 1632 | 9604 |
<I/σ(I)> | 11.4 | 51.38 | 0.73 |
Completeness [%] | 99.9 | 99.4 | 99.7 |
Redundancy | 6.799 | 6.296 | 7.052 |
CC(1/2) | 0.999 | 0.999 | 0.342 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine |