5S4U

Tubulin-Z30620520-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	104.880, 158.880, 178.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.560 - 2.390
R-factor	0.2126
Rwork	0.211
R-free	0.25190
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	62.560	62.560	2.450
High resolution limit [Å]	2.390	10.690	2.390
Rmerge	0.108	0.027	2.422
Rmeas	0.117	0.030	2.617
Total number of observations	801335
Number of reflections	118423	1469	8643
<I/σ(I)>	10.64	50.45	0.79
Completeness [%]	99.8	98.9	99.7
Redundancy	6.767	6.342	6.999
CC(1/2)	0.999	0.999	0.372

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine