5S4T

Tubulin-Z328695024-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	104.770, 158.420, 179.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.530 - 2.270
R-factor	0.2078
Rwork	0.206
R-free	0.24620
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	62.530	62.530	2.330
High resolution limit [Å]	2.270	10.150	2.270
Rmerge	0.091	0.032	2.469
Rmeas	0.098	0.035	2.663
Total number of observations	940983
Number of reflections	137603	1701	10053
<I/σ(I)>	11.21	47.71	0.7
Completeness [%]	99.8	98.9	99.7
Redundancy	6.838	6.325	7.097
CC(1/2)	0.999	0.998	0.340

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine