5S4S

Tubulin-Z240297434-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	105.550, 164.170, 173.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	86.680 - 2.350
R-factor	0.2459
Rwork	0.244
R-free	0.28360
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	86.680	86.680	2.410
High resolution limit [Å]	2.350	10.510	2.350
Rmerge	0.137	0.018	3.666
Rmeas	0.148	0.020	3.958
Total number of observations	835312
Number of reflections	124451	1556	9133
<I/σ(I)>	10.09	65.48	0.54
Completeness [%]	99.0	98.8	99
Redundancy	6.712	6.159	6.989
CC(1/2)	0.999	1.000	0.271

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine