5S4P

Tubulin-Z275165822-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	104.246, 157.049, 178.157
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	78.520 - 2.290
R-factor	0.2083
Rwork	0.208
R-free	0.24400
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	78.520	78.520	2.350
High resolution limit [Å]	2.290	10.240	2.290
Rmerge	0.130	0.100	1.748
Rmeas	0.142	0.110	1.898
Total number of observations	902641
Number of reflections	133558	1663	9780
<I/σ(I)>	7.3	17.45	1.04
Completeness [%]	100.0	99.4	100
Redundancy	6.758	6.14	6.597
CC(1/2)	0.990	0.985	0.504

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine