5S4N

Tubulin-Z285782452-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	105.260, 158.630, 179.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	90.810 - 2.530
R-factor	0.2178
Rwork	0.216
R-free	0.25780
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	90.810	90.810	2.600
High resolution limit [Å]	2.530	11.310	2.530
Rmerge	0.118	0.033	2.681
Rmeas	0.128	0.036	2.903
Total number of observations	676349
Number of reflections	100320	1255	7271
<I/σ(I)>	9.88	45.06	0.7
Completeness [%]	99.5	98.8	99.2
Redundancy	6.742	6.347	6.764
CC(1/2)	0.998	0.999	0.363

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine