5RHN
HISTIDINE TRIAD NUCLEOTIDE-BINDING PROTEIN (HINT) FROM RABBIT COMPLEXED WITH 8-BR-AMP
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-07 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 40.320, 40.320, 143.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.310 |
| R-factor | 0.162 |
| Rwork | 0.162 |
| R-free | 0.26400 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 25.300 * |
| Data reduction software | R-AXIS |
| Data scaling software | R-AXIS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.130 | 2.500 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.084 | |
| Total number of observations | 45935 * | |
| Number of reflections | 5578 | |
| <I/σ(I)> | 12 | |
| Completeness [%] | 98.0 | 97.3 |
| Redundancy | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.4 * | 30% POLYETHYLENE GLYCOL 8000, 0.1 M SODIUM ACETATE, 0.1 M SODIUM CACODYLATE, PH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 30 (%) | |
| 3 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 4 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
| 5 | 1 | drop | 8-Br-AMP | 0.2 (mM) | |
| 6 | 1 | drop | 150 (mM) | ||
| 7 | 1 | drop | Tris-Cl | 20 (mM) | pH7.4 |
