5QUM

Structure of unliganded HumRadA1.4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-04-02
Detector	Bruker Platinum 135
Wavelength(s)	1.514
Spacegroup name	P 21 21 21
Unit cell lengths	67.819, 69.501, 81.204
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.750 - 1.930
R-factor	0.2352
Rwork	0.232
R-free	0.28910
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.750	34.750	2.040
High resolution limit [Å]	1.930	5.730	1.930
Rmerge	0.082	0.024	1.927
Rmeas	0.089	0.026	2.108
Total number of observations	203005
Number of reflections	29549	1229	4543
<I/σ(I)>	14.68	36.89	0.94
Completeness [%]	99.3	99.2	96
Redundancy	6.87	6.669	5.972
CC(1/2)	0.999	1.000	0.638

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	50 mM Na/K phospate, 5% PEG1000H