5Q10
Ligand binding to FARNESOID-X-RECEPTOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 |
| Unit cell lengths | 93.957, 93.957, 48.020 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.680 - 2.200 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.950 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.600 | 2.290 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.112 | 0.399 |
| Number of reflections | 11238 | 648 |
| <I/σ(I)> | 9.11 | 1.5 |
| Completeness [%] | 90.3 | 46.8 |
| Redundancy | 3.65 | 0.61 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | evaporation, hanging drop | 8.5 | 298 | 0.2M MgCl2, 0.1M Tris pH 8.5, 25%PEG3350 |
