5OYM
HIV Integrase Binding Domain of Lens Epithelium-Derived Growth Factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.181, 54.814, 117.996 |
| Unit cell angles | 90.00, 91.23, 90.00 |
Refinement procedure
| Resolution | 14.980 - 2.050 |
| R-factor | 0.18506 |
| Rwork | 0.182 |
| R-free | 0.23628 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b4j |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.877 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.810 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmeas | 0.097 | 0.796 |
| Number of reflections | 55617 | 8059 |
| <I/σ(I)> | 8.6 | 1.7 |
| Completeness [%] | 97.1 | 97 |
| Redundancy | 2.4 | 2.4 |
| CC(1/2) | 0.510 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2 M Sodium Fluoride, 0.1 M bis-Tris propane, pH 8.5 and 20 % w/v PEG 3350 |
