5OYK
Outer-membrane expressed OmpT of Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2016-02-19 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.610, 74.330, 62.310 |
| Unit cell angles | 90.00, 96.74, 90.00 |
Refinement procedure
| Resolution | 46.410 - 3.200 |
| R-factor | 0.21381 |
| Rwork | 0.212 |
| R-free | 0.24435 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ehd |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.528 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.410 | |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 6356 | |
| <I/σ(I)> | 5 | 1.5 |
| Completeness [%] | 98.0 | 96 |
| Redundancy | 3.6 | 3.5 |
| CC(1/2) | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 15% PEG 4000 0.1 M sodium acetate 0.4 M ammonium thiocyanate |
