5OYD

GH5 endo-xyloglucanase from Cellvibrio japonicus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97948
Spacegroup name	P 21 21 21
Unit cell lengths	55.455, 97.598, 157.196
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	83.050 - 2.100
R-factor	0.22106
Rwork	0.218
R-free	0.28293
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5OYC.PDB
RMSD bond length	0.017
RMSD bond angle	1.793
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.050	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.062	0.547
Number of reflections	50763	4128
<I/σ(I)>	12.8
Completeness [%]	100.0	100
Redundancy	6.2	6.3
CC(1/2)	0.998	0.933

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	ammonium sulphate, MES buffer