5OY0
Structure of synechocystis photosystem I trimer at 2.5A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2017-03-18 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 212.170, 137.622, 225.095 |
| Unit cell angles | 90.00, 116.74, 90.00 |
Refinement procedure
| Resolution | 49.481 - 2.501 |
| R-factor | 0.2283 |
| Rwork | 0.228 |
| R-free | 0.26440 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.412 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((dev_2947: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.078 | |
| Number of reflections | 395994 | |
| <I/σ(I)> | 7.6 | 0.5 |
| Completeness [%] | 99.8 | 98.4 |
| Redundancy | 23.3 | 21.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | PEG 3350, magnesium chloride, tricine, glycine, magnesium acetate |
