5OWO
Human cytoplasmic Dynein N-Terminus dimerization domain at 1.8 Angstrom resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9793, 0.9792 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 101.242, 175.560, 50.444 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 87.780 - 1.790 |
| R-factor | 0.19811 |
| Rwork | 0.196 |
| R-free | 0.22924 |
| Structure solution method | MAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.035 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 44.290 |
| High resolution limit [Å] | 1.600 |
| Rmerge | 0.200 |
| Number of reflections | 119216 |
| <I/σ(I)> | 3.9 |
| Completeness [%] | 98.5 |
| Redundancy | 3.07 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291.15 | 100mM Sodium acetate, pH 5.5, 10% Glycerol, 50mM Calcium acetate, 20% PEG 2,000 MME |
