5OWF

Structure of a LAO-binding protein mutant with glutamine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-01
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.918400
Spacegroup name	P 21 21 21
Unit cell lengths	36.972, 58.730, 115.312
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.140 - 1.910
R-factor	0.191660731496
Rwork	0.190
R-free	0.23140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1laf
RMSD bond length	0.006
RMSD bond angle	0.760
Data reduction software	XDS (BUILT=20170615)
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.140	2.030
High resolution limit [Å]	1.910	1.910
Rmerge	0.174	1.954
Rpim	0.058	0.733
Number of reflections	20131	1946
<I/σ(I)>	8.7	1.12
Completeness [%]	99.5	97.49
Redundancy	9.7	7.5
CC(1/2)	0.995	0.375

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	150 mM potassium bromide, 30 % (wt/vol) PEG 2000 MME