5OW4

Crystal structure of a protease-resistant fragment of the Trypanosoma cruzi gamete fusion protein HAP2 ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	110
Detector technology	PIXEL
Collection date	2015-03-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00000
Spacegroup name	P 61 2 2
Unit cell lengths	150.090, 150.090, 123.780
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.130 - 3.100
R-factor	0.2344
Rwork	0.233
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mf1
RMSD bond length	0.008
RMSD bond angle	0.990
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.310
High resolution limit [Å]	3.100	3.100
Rmeas	0.201	2.493
Number of reflections	15453	2389
<I/σ(I)>	11.4	1.3
Completeness [%]	97.7	98.2
Redundancy	8.9	8.6
CC(1/2)	0.997	0.410

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	100mM CHES pH9.0 200mM NaCl 10%w/v PEG 8K