5OW0

Crystal structure of an electron transfer flavoprotein from Geobacter metallireducens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97929
Spacegroup name	P 21 21 21
Unit cell lengths	54.730, 72.750, 133.881
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.736 - 1.700
R-factor	0.138
Rwork	0.136
R-free	0.16740
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.539
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.630	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.092	0.584
Rpim	0.038	0.250
Number of reflections	59506	5846
<I/σ(I)>	15.9	3.9
Completeness [%]	99.7	99.6
Redundancy	12.8	2
CC(1/2)	0.998	0.918

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	Mother liquor: 15% (w/v) PEG 3350, 0.1 M HEPES, pH 7.5 mixed equal volumes of 10 mg/ml EtfAB in 300 mM NaCl, 20 mM HEPES, pH 7.5