5OUG
Humanized alpha-AChBP (acetylcholine binding protein) in complex with lobeline and allosteric binder fragment 4.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-10 |
| Detector | DECTRIS PILATUS3 X 1M |
| Wavelength(s) | 0.91983 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.673, 119.806, 84.543 |
| Unit cell angles | 90.00, 107.29, 90.00 |
Refinement procedure
| Resolution | 80.720 - 2.570 |
| R-factor | 0.2158 |
| Rwork | 0.214 |
| R-free | 0.24000 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.595 |
| Data reduction software | SCALA |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.610 | 2.670 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rpim | 0.083 | 1.350 |
| Number of reflections | 41656 | |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M HEPES pH 7.5, 8% ethylene glycol and 10% PEG8000. |
