5OTX
Extracellular domain of GLP-1 receptor in complex with GLP-1 variant Ala8Cys/Thr11Cys
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-18 |
| Detector | DECTRIS PILATUS3 R 1M |
| Wavelength(s) | 1.54187 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.870, 83.590, 42.830 |
| Unit cell angles | 90.00, 95.19, 90.00 |
Refinement procedure
| Resolution | 42.654 - 2.000 |
| R-factor | 0.1944 |
| Rwork | 0.192 |
| R-free | 0.23980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zgm |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.132 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2689: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.660 | 2.071 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.077 | 0.231 |
| Rpim | 0.032 | 0.223 |
| Number of reflections | 16996 | 1622 |
| <I/σ(I)> | 17.8 | 3.69 |
| Completeness [%] | 94.4 | 92.26 |
| Redundancy | 6.8 | 2 |
| CC(1/2) | 0.998 | 0.895 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Morpheus from Molecular Dimensions solution F2: 0.12 M Monosaccharides, 0.1 M Buffer system 1 pH 6.5, 20% (v/v) Ethylene Glycol, 10% (w/v) PEG8000 |
