5OTV
Extracellular domain of GLP-1 receptor in complex with GLP-1 variant Ala8Cyc/Thr11Hcs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-02 |
| Detector | DECTRIS PILATUS3 R 1M |
| Wavelength(s) | 1.54187 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.815, 83.998, 42.815 |
| Unit cell angles | 90.00, 95.52, 90.00 |
Refinement procedure
| Resolution | 42.616 - 2.000 |
| R-factor | 0.211 |
| Rwork | 0.208 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zgm |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.608 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2689: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.620 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.181 | 0.397 |
| Rmeas | 0.428 | |
| Rpim | 0.092 | 0.158 |
| Number of reflections | 17691 | |
| <I/σ(I)> | 8.5 | 5.8 |
| Completeness [%] | 98.0 | 91.98 |
| Redundancy | 4.6 | 7.04 |
| CC(1/2) | 0.962 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Morpheus from Molecular Dimensions, solution E5: 0.12 M Ethylene Glycols, 0.1 M Buffer System 2 pH 7.5, 205 (v/v) PEg500mme, 10% (w/v) PEG20000 |
