5OT2
RNA polymerase II elongation complex in the presence of 3d-Napht-A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.976 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 221.350, 394.930, 283.710 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.882 - 3.200 |
R-factor | 0.1789 |
Rwork | 0.178 |
R-free | 0.22180 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.033 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.882 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 202994 | |
<I/σ(I)> | 16.38 | |
Completeness [%] | 100.0 | |
Redundancy | 13.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 4-7% PEG 6000, 200 mM ammonium acetate, 300 mM sodium acetate, 50 mM HEPES pH 7.0, 5 mM TCEP |