5OT0
The thermostable L-asparaginase from Thermococcus kodakarensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-28 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 |
| Unit cell lengths | 70.740, 70.960, 107.730 |
| Unit cell angles | 72.13, 76.22, 87.83 |
Refinement procedure
| Resolution | 68.650 - 2.180 |
| R-factor | 0.19536 |
| Rwork | 0.194 |
| R-free | 0.22391 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wls |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.105 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.580 | 2.260 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.060 | 1.200 |
| Rmeas | 0.070 | 1.400 |
| Rpim | 0.750 | |
| Number of reflections | 98478 | 9841 |
| <I/σ(I)> | 10.06 | 1.3 |
| Completeness [%] | 97.4 | 97.2 |
| Redundancy | 3.5 | 3.6 |
| CC(1/2) | 1.000 | 0.590 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 294 | 0.09 M NPS, 0.1 M buffer system 2, pH 6.5-7.0, 28.5% (v/v) EDO_P8K from the Morpheus kit |
