5OSH

Structure of retromer VPS29-VPS35C subunits complexed with RidL N-terminal domain (1-236)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-21
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	90.201, 173.243, 445.775
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	222.890 - 4.300
R-factor	0.2566
Rwork	0.254
R-free	0.31090
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.374
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	autoSHARP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	222.890	222.890	3.190
High resolution limit [Å]	3.000	8.880	3.000
Rmerge	0.094	0.030	2.315
Rmeas	0.101	0.032	2.517
Total number of observations	651993
Number of reflections	93761	3671	14849
<I/σ(I)>	12.6	56.11	0.74
Completeness [%]	99.0	98.4	97.8
Redundancy	6.954	6.772	6.546
CC(1/2)	0.999	0.999	0.341

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	0.1 M sodium chloride 0.1 M Tris pH 8.0 4-8% PEG6000.