5OR3
Crystal structure of Aspergillus oryzae catechol oxidase in met/deoxy-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-22 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.627, 81.392, 82.200 |
| Unit cell angles | 87.19, 89.23, 73.89 |
Refinement procedure
| Resolution | 29.123 - 1.795 |
| R-factor | 0.1775 |
| Rwork | 0.176 |
| R-free | 0.20700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.735 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.130 | 1.900 |
| High resolution limit [Å] | 1.500 | 1.800 |
| Rmeas | 0.135 | |
| Number of reflections | 136838 | |
| <I/σ(I)> | 7.37 | 1.26 |
| Completeness [%] | 97.1 | 94.2 |
| Redundancy | 3.55 | |
| CC(1/2) | 0.601 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 294 | 6 % ethylene glycol, 10 % PEG 20000, 0.1 M sodium citrate |
