5OR1
BamA structure of Salmonella enterica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 146 |
| Detector technology | PIXEL |
| Collection date | 2014-08-11 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 124.037, 124.037, 131.480 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 107.420 - 2.920 |
| R-factor | 0.30884 |
| Rwork | 0.307 |
| R-free | 0.34703 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.430 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 130.000 |
| High resolution limit [Å] | 2.920 |
| Number of reflections | 13411 |
| <I/σ(I)> | 7.7 |
| Completeness [%] | 100.0 |
| Redundancy | 17.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1 M HEPES sodium (pH 7.5) and 1.0 M sodium citrate tribasic dehydrate |
