5OQR

Crystal structure of the S. pombe condensin Cnd3-Cnd2 subcomplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.977
Spacegroup name	P 21 21 21
Unit cell lengths	88.407, 142.113, 176.917
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.059 - 2.610
R-factor	0.2238
Rwork	0.223
R-free	0.25760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.568
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.060	2.670
High resolution limit [Å]	2.610	2.610
Rmerge	0.211	1.482
Rpim	0.060	0.425
Number of reflections	68083	4542
<I/σ(I)>	11.2	1.8
Completeness [%]	100.0	100
Redundancy	13.4	13
CC(1/2)	0.993	0.678

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		280	3 to 4% (w/v) PEG 4000, 0.1 M Na citrate pH 5.2 to 5.4, 0.2 M Na acetate