5OQP

Crystal structure of the S. cerevisiae condensin Ycg1-Brn1 subcomplex bound to DNA (crystal form I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-04
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.873
Spacegroup name	P 21 21 21
Unit cell lengths	80.140, 114.833, 155.211
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.171 - 2.980
R-factor	0.2214
Rwork	0.218
R-free	0.26170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5oqq
RMSD bond length	0.003
RMSD bond angle	0.547
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.171	3.140
High resolution limit [Å]	2.980	2.980
Rmerge	0.202	1.416
Rpim	0.084	0.572
Number of reflections	29952	4290
<I/σ(I)>	8.5	1.4
Completeness [%]	100.0	100
Redundancy	6.7	7.1
CC(1/2)	0.995	0.565

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		280	4% (w/v) PEG 4000 0.05 M Na citrate pH 6.6 5 mM MgSO4