5OQO

Crystal structure of the S. cerevisiae condensin Ycg1-Brn1 subcomplex bound to DNA (crystal form II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-04
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.873
Spacegroup name	P 21 21 21
Unit cell lengths	88.803, 117.940, 154.968
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.317 - 3.250
R-factor	0.2385
Rwork	0.236
R-free	0.27340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5oqp
RMSD bond length	0.003
RMSD bond angle	0.513
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.210	3.430
High resolution limit [Å]	3.250	3.250
Rmerge	0.271	1.854
Rpim	0.113	0.767
Number of reflections	26286	3796
<I/σ(I)>	6.7	1.1
Completeness [%]	100.0	100
Redundancy	6.7	6.8
CC(1/2)	0.995	0.513

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		280	5% (w/v) PEG 4000 0.05 M Na cacodylate pH 6.8 10 mM BaCl2