5OOU
Designed Ankyrin Repeat Protein (DARPin) YTRL-1 selected by directed evolution against Lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 103.500, 103.500, 96.130 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.817 - 2.104 |
| R-factor | 0.1864 |
| Rwork | 0.185 |
| R-free | 0.20630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5oos |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.484 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 65.557 | 65.557 | 2.140 |
| High resolution limit [Å] | 2.104 | 5.710 | 2.104 |
| Rmerge | 0.100 | 0.054 | 2.485 |
| Rmeas | 0.103 | 0.056 | 2.551 |
| Rpim | 0.024 | 0.014 | 0.569 |
| Total number of observations | 343934 | 16385 | 17894 |
| Number of reflections | 18216 | ||
| <I/σ(I)> | 16.5 | 40.5 | 1.4 |
| Completeness [%] | 100.0 | 100 | 98.9 |
| Redundancy | 18.9 | 15.7 | 20 |
| CC(1/2) | 0.999 | 0.999 | 0.475 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 298 | 1.5M AmSO4, 0.1M Tris pH=8.5, 12% glycerol |
