5OOS
Designed Ankyrin Repeat Protein (DARPin) YTRL-2 selected by directed evolution against Lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-01 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 102.487, 102.487, 96.714 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.390 - 2.260 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 65.393 | 65.393 | 2.267 |
| High resolution limit [Å] | 2.259 | 10.472 | 2.259 |
| Rmerge | 0.103 | 0.024 | 3.146 |
| Rmeas | 0.104 | 0.024 | 3.176 |
| Rpim | 0.014 | 0.004 | 0.431 |
| Total number of observations | 764056 | ||
| Number of reflections | 14609 | ||
| <I/σ(I)> | 33.4 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 52.3 | 36.1 | 53.4 |
| CC(1/2) | 1.000 | 1.000 | 0.674 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 298 | 30% PEG3000, 0.1M Tris pH=7, 0.2M NaCl |
