5OOR
Structure of CHK1 10-pt. mutant complex with staurosporine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2015-06-29 |
| Detector | BRUKER PHOTON 100 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.814, 65.699, 54.034 |
| Unit cell angles | 90.00, 101.64, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.900 |
| R-factor | 0.1666 |
| Rwork | 0.165 |
| R-free | 0.20780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5oop |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.265 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.770 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.108 | 0.474 |
| Number of reflections | 28254 | 4133 |
| <I/σ(I)> | 9.05 | 2.01 |
| Completeness [%] | 98.9 | 97.7 |
| Redundancy | 4 | 2.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 7% PEG 8000, 0.1 M MES buffer pH 6.5, 20% ethylene glycol |
