5ONF
The ENTH domain from epsin-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-12-06 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.127 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 68.929, 119.363, 129.542 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.110 - 2.800 |
R-factor | 0.27236 |
Rwork | 0.272 |
R-free | 0.28470 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.050 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 27.000 |
High resolution limit [Å] | 2.750 |
Number of reflections | 13379 |
<I/σ(I)> | 11.8 |
Completeness [%] | 99.1 |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.2 M CaCl2, 25 % PEG 3350 and 0.1 M Tris pH 8.5. |