5OLP
Galacturonidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-21 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9163 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 127.545, 127.545, 263.641 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.730 - 2.000 |
R-factor | 0.18341 |
Rwork | 0.182 |
R-free | 0.21534 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.510 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | SHELXCD |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.730 | 2.040 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 73408 | 4477 |
<I/σ(I)> | 12.8 | 1.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 26.8 | 28 |
CC(1/2) | 0.998 | 0.620 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 mM sodium chloride, 100 mM Bis-Tris buffer pH 5.5 and 25% PEG 3350 |