5OLC
Crystal structure of the 3,6-anhydro-D-galactonate cycloisomerase from Zobellia galactanivorans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-30 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.840, 154.070, 150.870 |
| Unit cell angles | 90.00, 104.38, 90.00 |
Refinement procedure
| Resolution | 49.170 - 2.790 |
| R-factor | 0.233 |
| Rwork | 0.231 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hpn |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.190 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.170 | 2.870 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Number of reflections | 93118 | |
| <I/σ(I)> | 3.97 | 0.87 |
| Completeness [%] | 99.0 | 95.6 |
| Redundancy | 3.6 | 3.6 |
| CC(1/2) | 0.978 | 0.473 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | Optimized conditions were a 1:1 ratio of 0.2 M tri potassium citrate, 20% PEG 3350 to 25 mg/ml ZGAL_3156. |
