5OJX

Crystal structure of regulator protein 2 (PamR2) from the pamamycin biosynthetic gene cluster of Streptomyces alboniger

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-13
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.918400
Spacegroup name	C 1 2 1
Unit cell lengths	98.270, 56.030, 39.270
Unit cell angles	90.00, 97.99, 90.00

Refinement procedure

Resolution	38.889 - 2.100
R-factor	0.2298
Rwork	0.227
R-free	0.27660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2y30
RMSD bond length	0.007
RMSD bond angle	0.912
Data reduction software	XDS (20170615)
Data scaling software	XSCALE (20170615)
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.889	38.889	2.200
High resolution limit [Å]	2.100	10.000	2.100
Rmerge	0.036	0.016	0.717
Rmeas	0.041	0.019	0.816
Number of reflections	12407	118	1614
<I/σ(I)>	20.02	67.89	1.86
Completeness [%]	99.6	93.7	100
Redundancy	4.288	3.881	4.473
CC(1/2)	1.000	1.000	0.708

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	5mg/mL PamR2 150 mM NaCl 722mM tri-Lithium citrate