5OJW
S. cerevisiae UBC13 - MMs2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-22 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.070989 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.425, 66.281, 126.614 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.780 - 2.000 |
| R-factor | 0.1626 |
| Rwork | 0.160 |
| R-free | 0.20540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jat |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.035 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.780 | 1.820 |
| High resolution limit [Å] | 1.773 | 1.773 |
| Rmerge | 0.071 | |
| Rpim | 0.056 | |
| Number of reflections | 29813 | |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 97.6 | |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 292 | 0.1M Bis-Tris Propane / Citric Acid Buffer, pH 7.6, 20% PEG3000 |
