5OJG
Crystal structure of the dehydrogenase/reductase SDR family member 4 (DHRS4) from Caenorhabditis elegans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-05-21 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.976261 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 93.600, 93.600, 132.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 76.350 - 1.900 |
R-factor | 0.18133 |
Rwork | 0.179 |
R-free | 0.22344 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3o4r |
RMSD bond length | 0.016 |
RMSD bond angle | 1.814 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 76.350 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rpim | 0.489 | 5.039 |
Number of reflections | 46906 | 3280 |
<I/σ(I)> | 8.3 | |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 23.3 | |
CC(1/2) | 0.995 | 0.214 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 10 % (w/v) PEG 8000 200 mM NaCl 100 mM K/Na-phospate pH 6.2 |