5OJ2
Crystal structure of the chicken MDGA1 ectodomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2013-04-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97938 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 103.180, 109.040, 208.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 96.651 - 3.200 |
| R-factor | 0.2163 |
| Rwork | 0.214 |
| R-free | 0.25140 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.986 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2044)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.651 | 3.280 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmeas | 0.155 | 1.415 |
| Number of reflections | 39616 | 2874 |
| <I/σ(I)> | 13.6 | 1.8 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 11 | 10.9 |
| CC(1/2) | 0.622 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M HEPES pH7.5, 3% w/v polyethylene glycol 8000 |
