5OHZ
Crystal structure of Mycolicibacterium hassiacum glucosylglycerate hydrolase (MhGgH) - SeMet derivative
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97909, 0.97924 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.750, 86.101, 159.720 |
| Unit cell angles | 90.00, 93.03, 90.00 |
Refinement procedure
| Resolution | 57.421 - 2.036 |
| R-factor | 0.2012 |
| Rwork | 0.199 |
| R-free | 0.24460 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.099 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.421 | 2.070 |
| High resolution limit [Å] | 2.036 | 2.040 |
| Rmerge | 0.105 | 0.637 |
| Rmeas | 0.128 | 0.776 |
| Rpim | 0.072 | 0.438 |
| Number of reflections | 255146 | |
| <I/σ(I)> | 7.6 | 1.6 |
| Completeness [%] | 96.5 | 82.1 |
| Redundancy | 2.8 | 2.8 |
| CC(1/2) | 0.990 | 0.659 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Tris-Bicine pH 8.5, amino acids, PEG 4000, glycerol |
