5OHU

The X-ray Structure of Lytic Transglycosylase Slt from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	P 63
Unit cell lengths	163.981, 163.981, 56.706
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.337 - 2.200
R-factor	0.1843
Rwork	0.182
R-free	0.23780
Structure solution method	AB INITIO PHASING
RMSD bond length	0.007
RMSD bond angle	0.877
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.340	2.280
High resolution limit [Å]	2.200	2.200
Rpim	0.050	0.860
Number of reflections	44540
<I/σ(I)>	14.8	1.35
Completeness [%]	100.0	100
Redundancy	19.4	19.3
CC(1/2)	0.990	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	K/Na phosphate 1M