5OHO
Crystal structure of the KOWx-KOW4 domain of human DSIF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00003056744 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.286, 54.403, 73.065 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.635 - 1.601 |
R-factor | 0.1767 |
Rwork | 0.174 |
R-free | 0.22130 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.862 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.635 | 43.635 | 1.700 |
High resolution limit [Å] | 1.600 | 4.780 | 1.600 |
Rmerge | 0.047 | 0.027 | 1.336 |
Rmeas | 0.051 | 0.029 | 1.452 |
Number of reflections | 50856 | 1915 | 8120 |
<I/σ(I)> | 15.88 | 53.72 | 0.94 |
Completeness [%] | 99.7 | 99.8 | 98.7 |
Redundancy | 6.76 | 6.81 | 6.466 |
CC(1/2) | 0.999 | 0.999 | 0.485 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M sodium cacodylate pH 6.5, 1.0 M tri-sodium citrate dihydrate |