5OHN

Crystal structure of USP30 in covalent complex with ubiquitin propargylamide (low resolution)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.928190
Spacegroup name	P 65
Unit cell lengths	181.834, 181.834, 94.957
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	157.473 - 3.600
R-factor	0.2174
Rwork	0.211
R-free	0.25260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ohk
RMSD bond length	0.020
RMSD bond angle	1.619
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12rc2_2821: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	157.470	3.940
High resolution limit [Å]	3.600	3.600
Rmerge	0.124	0.900
Rpim	0.072	0.523
Number of reflections	20776	4948
<I/σ(I)>	10	1.9
Completeness [%]	99.3	99.6
Redundancy	7	7.2
CC(1/2)	0.997	0.593

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	20% (w/v) PAA 5100 Na, 100 mM Hepes pH 8.0, 2.5% (v/v) glycerol