5OH2

Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with Pyrrolidin-2-one (Butyrolactam)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	56.911, 59.686, 88.443
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.400 - 1.900
R-factor	0.1789
Rwork	0.177
R-free	0.22474
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2V2Y
RMSD bond length	0.012
RMSD bond angle	1.537
Data reduction software	XDS
Data scaling software	XDS
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.400	2.010
High resolution limit [Å]	1.900	1.900
Rmerge	0.050	0.490
Number of reflections	24410	3836
<I/σ(I)>	12.5	1.89
Completeness [%]	98.4	96.7
Redundancy	2.86	2.81
CC(1/2)	1.000	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.1 M sodium acetate pH 4.6, 15 %(W/V) PEG 20000