5OH2
Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with Pyrrolidin-2-one (Butyrolactam)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-08 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.911, 59.686, 88.443 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.400 - 1.900 |
| R-factor | 0.1789 |
| Rwork | 0.177 |
| R-free | 0.22474 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2V2Y |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.537 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.400 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.050 | 0.490 |
| Number of reflections | 24410 | 3836 |
| <I/σ(I)> | 12.5 | 1.89 |
| Completeness [%] | 98.4 | 96.7 |
| Redundancy | 2.86 | 2.81 |
| CC(1/2) | 1.000 | 0.790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1 M sodium acetate pH 4.6, 15 %(W/V) PEG 20000 |
