5OGL

Structure of bacterial oligosaccharyltransferase PglB in complex with an acceptor peptide and an lipid-linked oligosaccharide analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-30
Detector	PSI PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	83.940, 116.180, 172.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.767 - 2.700
R-factor	0.2185
Rwork	0.217
R-free	0.24390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rce
RMSD bond length	0.009
RMSD bond angle	1.117
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.767	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.087	1.358
Rmeas	0.091
Number of reflections	46914
<I/σ(I)>	21.5	2.18
Completeness [%]	100.0	100
Redundancy	12.8	13.5
CC(1/2)		0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	9.4	293	0.1M glycine pH 9.4, 0.12M lithium sulfate, 29-31% PEG400