5OFX
Plla lectin, trisaccharide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-07 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.976251 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.979, 62.998, 75.860 |
| Unit cell angles | 101.15, 112.79, 94.44 |
Refinement procedure
| Resolution | 44.090 - 1.750 |
| R-factor | 0.1766 |
| Rwork | 0.175 |
| R-free | 0.20570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l7l |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.738 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.090 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.163 | 0.773 |
| Rmeas | 0.846 | |
| Rpim | 0.340 | |
| Number of reflections | 89118 | |
| <I/σ(I)> | 8.1 | 2 |
| Completeness [%] | 90.7 | 91.9 |
| Redundancy | 6.1 | 6 |
| CC(1/2) | 0.774 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Magnesium acetate, 20 % w/v PEG 3350 |
